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BulletApplications with the Incoatec Microfocus Source IμS

 

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IµS for Cu radiation

The brightest sealed tube on earth!


Protein Crystallography with IµS for Cu

IµS delivers a two-dimensional focused beam with an unprecedented flux of > 3.0 x 108 cps Cu-Kα radiation giving a performance beyond traditional 5 kW rotating anode systems with second generation multilayer mirrors, for example Montel 200 or Osmic Blue mirrors. The excellent beam stability guarantees high data quality allowing S-SAD phasing. The beam cross-section of the image focus is about 250 µm (FWHM). The dedicated and patented mirror housing allows easy and reliable alignment. An optional variable aperture at the exit of the housing enables the reduction of the beam divergence to resolve long unit cell axes.

Glucose Isomerase
crystal size [µm³] 240x240x150
exposure time 40 s/image
total time 43 h
diffraction limit [Å] 1.50
anomalous signal limit [Å] 2.7 (Ca2+, 9 S)
mean I/σ 21.8 (3.5)*
redundancy 14.4 (4.5)*
R (int) 0.0652
R (p.i.m.) 0.0149
R1(I>2σ(I); all) 0.1587; 0.2047

(* values for outer resolution shell 1.60 ? 1.50 Å)

 

 

 

 

The table shows statistical quantities of a single crystal measurement of glucose isomerase (a = 93.88 Å, b = 99.68 Å, c = 102.90 Å; I222; T = 100 K; 388 amino acids / ASU). Ca2+ site has been found in the initial phasing with SHELXD. A typical diffraction pattern of glucose isomerase is shown left.

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